Table 2.
Authentication and contaminant detection of three cell line pairs with serial dilutionsa
| Cell line mixture | Major component | Minor component (contaminant) | Minor component ratio (percentage)b | No. of informative SNPs | Heterogeneity ratio (percentage) | Major component inferredc | Minor component (contaminant) inferredd | Contamination ratio (percentage)c | P-valuee |
|---|---|---|---|---|---|---|---|---|---|
| PANC-1:RT4 | PANC-1 | 118 | 1.4 | PANC-1 (99.46%) | |||||
| RT4 | 122 | 1.65 | RT4 (98.54%) | ||||||
| RT4 | PANC-1 | 5 | 86 | 3.3 | RT4 (97.78%) | PANC-1 (96.73%) | 2.88 | 2.98E−16 | |
| RT4 | PANC-1 | 2.5 | 80 | 1.85 | RT4 (97.93%) | PANC-1 (94.79%) | 1.08 | 6.65E−12 | |
| RT4 | PANC-1 | 1.25 | 81 | 1.1 | RT4 (97.76%) | PANC-1 (88.14%) | 0.41 | 3.97E−09 | |
| RT4 | PANC-1 | 0.625 | 80 | 1.06 | RT4 (97.76%) | ||||
| PANC-1 | RT4 | 5 | 131 | 8.09 | PANC-1 (99.50%) | RT4 (98.33%) | 7.21 | 5.01E−17 | |
| PANC-1 | RT4 | 2.5 | 128 | 4.06 | PANC-1 (99.35%) | RT4 (95.50%) | 2.81 | 5.01E−17 | |
| PANC-1 | RT4 | 1.25 | 132 | 2.75 | PANC-1 (99.49%) | RT4 (90.73%) | 1.48 | 5.01E−17 | |
| PANC-1 | RT4 | 0.625 | 139 | 2.62 | PANC-1 (99.47%) | RT4 (81.61%) | 1.08 | 1.67E−08 | |
| LNCAPCLONEFGC:MV-4-11 | LNCAPCLONEFGC | 97 | 1.005 | LNCAPCLONEFGC (99.03%) | |||||
| MV-4-11 | 93 | 0.965 | MV-4-11 (99.03%) | ||||||
| MV-4-11 | LNCAPCLONEFGC | 5 | 99 | 9 | MV-4-11 (99.45%) | LNCAPCLONEFGC (96.83%) | 9.08 | 5.01E−17 | |
| MV-4-11 | LNCAPCLONEFGC | 2.5 | 111 | 4.51 | MV-4-11 (99.50%) | LNCAPCLONEFGC (98.34%) | 4.14 | 5.01E−17 | |
| MV-4-11 | LNCAPCLONEFGC | 1.25 | 117 | 2.18 | MV-4-11 (99.18%) | LNCAPCLONEFGC (90.32%) | 2.01 | 1.67E−09 | |
| MV-4-11 | LNCAPCLONEFGC | 0.625 | 112 | 1.58 | MV-4-11 (99.00%) | LNCAPCLONEFGC (89.44%) | 1.48 | 1.67E−09 | |
| LNCAPCLONEFGC | MV-4-11 | 5 | 102 | 2.35 | LNCAPCLONEFGC (98.99%) | MV-4-11 (94.57%) | 2.14 | 5.01E−17 | |
| LNCAPCLONEFGC | MV-4-11 | 2.5 | 101 | 1.67 | LNCAPCLONEFGC (99.04%) | MV-4-11 (91.58%) | 0.88 | 2.37E−11 | |
| LNCAPCLONEFGC | MV-4-11 | 1.25 | 98 | 1.49 | LNCAPCLONEFGC (99.03%) | MV-4-11 (87.77%) | 0.71 | 2.76E−11 | |
| LNCAPCLONEFGC | MV-4-11 | 0.625 | 105 | 1.36 | LNCAPCLONEFGC (99.03%) | ||||
| CAL28:RAJI | CAL27 | 39 | 1.39 | CAL27 (97.39%) | |||||
| RAJI | 114 | 1.18 | RAJI (98.56%) | ||||||
| RAJI | CAL27 | 5 | 116 | 5.36 | RAJI (98.66%) | CAL27 (99.12%) | 5.54 | 5.01E−17 | |
| RAJI | CAL27 | 2.5 | 127 | 4.17 | RAJI (98.32%) | CAL27 (94.70%) | 4.01 | 8.37E−11 | |
| RAJI | CAL27 | 1.25 | 121 | 1.84 | RAJI (98.51%) | CAL27 (90.83%) | 2.21 | 8.37E−06 | |
| RAJI | CAL27 | 0.625 | 116 | 2.49 | RAJI (98.50%) | CAL27 (90.43%) | 2.28 | 1.67E−07 | |
| CAL27 | RAJI | 5 | 121 | 7.11 | CAL27 (99.17%) | RAJI (99.51%) | 5.41 | 5.01E−17 | |
| CAL27 | RAJI | 2.5 | 113 | 3.79 | CAL27 (98.94%) | RAJI (92.06%) | 2.41 | 4.30E−13 | |
| CAL27 | RAJI | 1.25 | 112 | 2.14 | CAL27 (98.61%) | RAJI (83.42%) | 1.21 | 4.20E−07 | |
| CAL27 | RAJI | 0.625 | 112 | 1.4 | CAL27 (98.49%) | RAJI (83.75%) | 0.61 | 1.59E−07 |
aAverage values for each cell line mixture with three technical replicates, except the unmixed ones (see Supplementary Table S2 for full data).
bPercentage of the minor cell line based on cell counts.
cGenotype similarity shown in parentheses.
dChimeric genotype similarity shown in parentheses.
eProbability that the inferred minor component is incorrect.