Table 3.
Authentication and contaminant detection of 22 cell line mixtures
| Cell line mixturea | No. of informative SNPs | Heterogeneity ratio (percentage) | Major component inferredb | Minor component (contaminant) inferredc | Contamination ratio (percentage) | P-valued |
|---|---|---|---|---|---|---|
| ME180:143B | 119 | 6.54 | ME180 (98.06%) | 143B (97.09%) | 7.01 | 5.01E−17 |
| 143B:ME180 | 135 | 3.24 | 143B (98.55%) | ME180 (94.17%) | 3.21 | 5.01E−17 |
| JEG-3:A-875 | 104 | 1.63 | JEG-3 (98.49%) | A-875 (87.94%) | 1.21 | 5.01E−13 |
| A-875:JEG-3 | 93 | 15.50 | A-875 (91.71%) | JEG-3 (99.00%) | 16.71 | 5.01E−17 |
| HT3:C33A | 115 | 3.54 | HT3 (100%) | C33A (97.06%) | 3.41 | 5.01E−17 |
| C33A:HT3 | 90 | 4.34 | C33A (99.01%) | HT3 (100%) | 4.61 | 0 |
| DOTC24510:CASKI | 136 | 5.47 | DOTC24510 (98.99%) | CASKI (93.97%) | 5.21 | 5.01E−17 |
| CASKI:DOTC24510 | 129 | 4.26 | CASKI (98.98%) | DOTC24510 (91.84%) | 4.11 | 5.01E−17 |
| HLE:HCC94 | 163 | 2.62 | HLE (99.0%), HLF (96.08%) | HCC94 (91.46%) | 2.91 | 5.01E−17 |
| HCC94:HLE | 133 | 10.65 | HCC94 (97.6%) | HLE (96.63%), HLF (96.63%) | 10.11 | 5.01E−17 |
| NCIH1993:LS174T | 141 | 3.97 | NCIH1993 (98.05%) | LS174T (95.12%), LS180 (95.12%), HM7 (94.63%) | 4.21 | 5.01E−17 |
| LS174T:NCIH1993 | 114 | 4.88 | LS174T (99.02%), LS180 (99.03%), HM7 (98.54%) | NCIH1993 (97.06%) | 4.71 | 5.01E−17 |
| OSC19:SF763 | 152 | 7.03 | OSC19 (98.08%) | SF763 (96.15%) | 5.71 | 5.01E−17 |
| SF763:OSC19 | 133 | 3.35 | SF763 (99.02%) | OSC19 (90.15%) | 2.91 | 2.51E−16 |
| SW626:SJCRH30 | 155 | 11.67 | SW626 (95.63%) | SJCRH30 (98.54%) | 13.21 | 5.01E−17 |
| SJCRH30:SW626 | 88 | 1.79 | SJCRH30 (98.55%) | SW626 (94.2%) | 2.01 | 1.58E−16 |
| A-875:ME180 | 115 | 2.68 | A-875 (98.56%) | ME180 (95.67%) | 2.31 | 5.01E−17 |
| DOTC24510:CASKI | 144 | 1.75 | DOTC24510 (98.5%) | CASKI (86%) | 1.71 | 1.00E−15 |
| OSC19:SF763 | 130 | 2.68 | OSC19 (98.56%) | SF763 (93.27%) | 2.11 | 5.01E−17 |
| NOZ:SW626 | 127 | 0.82 | NOZ (97.56%) | SW626 (82.93%) | 0.71 | 3.98E−11 |
| SNU739:MM1R | 121 | 2.29 | SNU739 (99.01%) | MM1R (94.03%), MM1S (94.03%) | 1.71 | 5.01E−17 |
| U251:SR | 127 | 1.09 | U251 (98.54%) | SR (89.76%) | 1.11 | 5.01E−17 |
aIn the format of major cell line:minor/contaminating cell line.
bGenotype similarity shown in parentheses.
cChimeric genotype similarity shown in parentheses.
dProbability that the inferred minor component is wrong.