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. 2020 Nov;23(11):1396–1400. doi: 10.22038/IJBMS.2020.44853.10463

Table 1.

Primers used in this study for detection of resistance genes among P. aeruginosa and A. baumannii isolates

Gene Primer sequencing 5’ 3’ PCR product size (bp) Tm (oC) Reference
kpc-F CTGTCTTGTCTCTCATGGCC 636 57.98 [12]
kpc-R CCTCGCTGTGCTTGTCATCC 61.36
ges -F GTTTTGCAATGTGCTCAACG 371 57.09 [13]
ges-R TGCCATAGCAATAGGCGTAG 57.54
vim -F GATGGTGTTTGGTCGCATA 390 55.61 [14]
vim -R CGAATGCGCAGCACCAG 59.54
imp -F TTGACACTCCATTTACDG a 139 48.56 [14]
imp-R GATYGAGAATTAAGCCACYCT a 51.92
NDM-1 -F CCCGGCCACACCAGTGACA 129 64.73 [14]
NDM-1-R GTAGTGCTCAGTGTCGGCAT 60.11
SPM-1- F GGGTGGCTAAGACTATGAAGCC 447 60.49 [14]
SPM-1-R GCCGCCGAGCTGAATCGG 63.90
oxa-48-F CCAAGCATTTTTACCCGCATCKACC 389 63.21 [15]
oxa-48-R GYTTGACCATACGCTGRCTGCG 62.30
oxa-23- F GATGTGTCATAGTATTCGTCGT 1058 55.86 [6]
oxa-23- R TCACAACAACTAAAAGCACTGT 56.69
oxa-40- F GGAATTCCATGAAAAAATTTATACTTCC 846 56.44 [17]
oxa-40 - R CGGGATCCCGTTAAATGATTCCAAGATTTTCTAGCG 68.57
oxa-24- F GGTTAGTTGGCCCCCTTAAA 246 57.39 [18]
oxa-24- R AGTTGAGCGAAAAGGGGATT 57.41
oxa-58- F AAGTATTGGGGCTTGTGCTG 598 58.45 [18]
oxa-58- R CCCCTCTGCGCTCTACATAC 59.68
oxa-51- F TAATGCTTTGATCGGCCTTG 353 56.48 [18]
oxa-51- R TGGATTGCACTTCATCTTGG 56.01
b AdeB - F AACGGACGACCATCTTTGAGTATT 84 60.32 [36]
b AdeB - R CAGTTGTTCCATTTCACGCATT 58.36
b16srRNA-F CAGCTCGTGTCGTGAGATGT 151 60.11 [37]
b 16srRNA-R CGTAAGGGCCATGATGACTT 57.67
b MexX-F TGAAGGCGGCCCTGGACATCAGC 326 69.22 [2]
b MexX-R GATCTGCTCGACGCGGGTCAGCG 69.78
b MexA-F CGACCAGGCCGTGAGCAAGCAGC 316 70. 52 [2]
b MexA-R GGAGACCTTCGCCGCGTTGTCGC 70.42
b MexC-F GTACCGGCGTCATGCAGGGTTC 164 65.93 [2]
b MexC-R TTACTGTTGCGGCGCAGGTGACT 67.14