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PCR conditions used in this study for detection of carbapenem-resistant genes
| Number of cycles | Time | Temperature ( o C) | Cycle |
|---|---|---|---|
| 1 | 1-10a min | 94 | Initial denaturation |
| 30-40 | 30- 45a sec | 94 | Denaturation Annealing Extension |
| 30- 40a sec | 54- 63a | ||
| 30 sec-1a min | 72 | ||
| 1 | 1-7a min | 72 | Final extension |
a based on each gene
