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Immunofluorescence staining of α-actin in the hippocampus of rats. (A) Nuclei staining by DAPI and antibody to α-actin and merge of them from each group (magnification, ×400). (B), The percentage of positive reaction in each group. Red arrows point to α-actin positive cells. Scale Bars: 20 µm. Ratio of α-actin positive neurons is displayed as the mean±SD (n=4 per groups); (***P<0.001)
