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. 2020 Nov 13;13:897–911. doi: 10.2147/JIR.S281393

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© 2020 Yang et al.

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Syk affinitive components from PLE decreased the expression and phosphorylation of Syk and its downstream signaling proteins in vitro. (A) The expression of Syk were determined by immunofluorescence staining, (a) representative photomicrographs (×200) of Syk in RBL-2H3 cells and (b) their relative fluorescence intensities (n = 3). (B) The expression of p-Syk were determined by immunofluorescence staining, (a) representative photomicrographs (×200) of Syk in RBL-2H3 cells and (b) their relative fluorescence intensities (n = 3). (C–J) The expression of Syk, p-Syk, PKC, p-PKC, p65, p-p65, cPLA₂, and p-cPLA₂ in DNP-IgE/BSA-induced RBL-2H3 cells as determined by WB (n = 5). Data were shown as mean ± SEM. ^##P < 0.01 vs control group, *P < 0.05 and **P < 0.01 vs model group.