Figure 6.

Verification that Syk, PKC, NF-κB and cPLA₂ were associated with Mix attenuated Inflammation in DNP-IgE/BSA-induced RBL-2H3 cells. (A) (a) Images of DNP-IgE/BSA-induced RBL-2H3 cells stained by toluidine blue (magnified ×400), (b) the percent of degranulation (%) calculated (n = 3), (c) the release rate of β-Hex (%) and (d) the level of TNF-α in cells co-treated Syk affinitive compounds with Syk inhibitor (n = 3 independent experiments). (B) The release rate of β-Hex and (C) production of TNF-α in cells co-treated Syk affinitive compounds with PKC, NF-κB or cPLA₂ inhibitors (n = 3 independent experiments). Data were shown as mean ± SEM. ^##P < 0.01 vs control group; **P < 0.01 vs model group.