Figure 6.

In vitro eotaxin-1 inhibition assay on HFL-1 cells. (A) Dose-dependent inhibitory effect of ILQ, ILQ-SMEDDS and blank SMEDSS (equivalent volume to ILQ-SMEDDS) on eotaxin-1 production by HFL-1 cells. Results were expressed as mean ± SD.* or ^#P <0.05; **or ^##P <0.01; ***or ^###P <0.001 (n=3), compared to the negative control. (B) Cell viability of HFL-1 cells at various concentrations of ILQ suspension, ILQ-SMEDDS and blank SMEDDS. **p < 0.01, ***p < 0.001 (n=3). (C and D) Percentage of eotaxin-1 inhibition vs log(x) ILQ-SMEDDS (C) and ILQ (D) concentration curve. HFL-1 cells (2×10⁵ cells/mL) were cultured with ILQ, ILQ-SMEDD and blank SMEDDS at concentrations 0.63, 1.25, 2.5, 5, 10 and 15 μg/mL for 3 days. The supernatants were harvested and eotaxin-1 levels were measured by enzyme-linked immunosorbent assay.