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(A) Schematic of the FTSM comprising epidermis and dermis cultured in (B) cell culture insert on a porous membrane. (C) Histological Hematoxylin and Eosin staining of FTSM in E10 skin medium (3D-1) compared to (D) 3D-3 and (E) 3D-4, the best experimental co-culture media that supported L4 fitness. Scale bar = 100 μm. (1) porous membrane, (2) outer-well medium, (3) dermis, (4) epidermis.
