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. 2020 Nov 17;15(11):e0241840. doi: 10.1371/journal.pone.0241840

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© 2020 Li et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Flow cytometry analysis of CD45⁺CD11b⁺F4/80⁺CD206⁺ M2 macrophages in colonic tissues from AOM/DSS-treated group (AOM/DSS) and AOM/DSS-treated with H. pylori infection group (Hp+AOM/DSS) (n = 6/group). (B) Representative immunohistochemical images of the M2 marker CD206 staining in tumor tissues (×400) and (C) the numbers of CD206⁺ cells per field (n = 6/group). (D) Relative mRNA expression of M2-associated genes (Arginase1, CD206, Trem2 and Ym1) in colonic tumors (n = 5-6/group). (E) Arginase1, Ym1, CD206 and Trem2 mRNA expression in F4/80⁺ macrophages isolated from tumors of AOM/DSS group and Hp+AOM/DSS group (n = 5-6/group). Data are represented as mean ± SEM. *P < 0.05.