Figure 2.

ED-71 reduces LPS-induced cell death in HGFs. Part of cells were only treated by ED-71 (0, 0.5, 5 and 50 nM) for 24 h. Another part of cells were pretreated by ED-71 (0, 0.5, 5 and 50 nM) for 24 h, followed by 5 μg/mL LPS for 6 h. (A) The chemical formula of ED-71. (B) Cell viability were detected by CCK8. (C) The LDH released in HGFs supernatant were detected by LDH assay kit. Data were demonstrated as mean ± SEM from three experiments independently. ***P < 0.001 compared with control group. ^##P < 0.01, ^#P < 0.05 compared with the group only treated by 5 μg/mL LPS.