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. 2020 Nov 13;14:4901–4913. doi: 10.2147/DDDT.S269223

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© 2020 Huang et al.

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The anti-pyroptotic ability of ED-71 was Nrf2/HO-1-dependent. After treating by 5 μM ML385 for 3 h, cells were the treated by 5 nM ED-71 for 24 h and then incubated with 5 μg/mL LPS for 6 h. (A) Cell viability and (B) LDH release were detected by CCK8 and LDH assay kit respectively. (C and D) The relative proteins expression of TLR4, Nrf2, HO-1. (E) Relative mRNA expression in HGFs. (F and G) pyroptosis- associated proteins were detected by Western-blot. (H) Relative mRNA expression was shown. (I and J) The expression of IL-6 and IL-8. (K and L) The intercellular ROS and H2O2 content in HGFs. (M) Flow cytometry of propidium iodide and annexin V (FITC)-stained HGFs. The data collected from three independent experiments were presented as mean ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05 compared with control group. ^###P < 0.001, ^##P < 0.01 and ^#P < 0.05 compared with the group only treated by 5 μg/mL LPS. ^&&P < 0.01 and ^&P < 0.05 compared with 5 nM ED-71 treated and then stimulated by 5 μg/mL LPS group.