Fig 4. Effect of PMS on the growth of norfloxacin treated cells.
The E. coli wt (BE1000) (A), ΔiscUA (AG030) (B) and ΔsufABCDSE (AG031) (C) strains were grown to mid-log phase in LB and then diluted to inoculate fresh LB medium (1 x 105 c.f.u./mL), in 96-well microplate, supplemented or not (circles) with norfloxacin (final concentrations; 120 ng/mL squares; 140 ng/mL triangles; 160 ng/mL crosses) in the presence (right panels) or not (left panels) of PMS (1/16 of the MICPMS of each strains; MICPMS were 140 μM for wt; 160 μM for ΔiscUA; 55 μM for ΔsufABCDSE). Cultures were incubated at 37°C with shaking. Plates were read for OD600 in Tecan Infinite. The experiments were repeated at least three times. Complete growth curves of a representative experiment are shown. Corresponding growth parameters are indicated in Table 3.