Figure 2:

Administration of FZHY compound attenuates BDL-induced liver fibrosis.

Development of liver fibrosis was evaluated in sham- or BDL-operated mice ± FZHY (4.0 g/kg body weight, n=10 per group). (A) Sirius Red staining (×4) and immunofluorescence staining for α-SMA ⁺and Desmin⁺ myofibroblasts, representative images are taken using objectives x4 and x20, respectively. (B) Serum ALT levels (IU/L) and Hepatic hydroxyproline content (ng/mg liver) were measured. The positive area for Hepatic Sirius Red staining, immunostaining for α-SMA (red) and Desmin (green) calculated as percent, *p<0.05, **p<0.01. (C) Hepatic levels of hepatic mRNA expression of pro-fibrogenic genes were measured using quantitative RT-PCR *p<0.05, **p<0.01.