Figure 3:

FZHY compound possesses hepatoprotective effect.

Murine hepatocytes were isolated from uninjured C57BL6 mice, plated and cultured ±CCl₄ (5mM) ± FZHY (0.08 or 1.25 μg/μl). (A) The effect of FZHY on viability of cultured primary hepatocytes was evaluated by the number of live cells, shown as percent, compared with hepatocytes cultured in the presence of vehicle (PBS), *p<0.05, **p<0.01. (B) mRNA expression levels of Bax, Bcl-2, or Bcl-XI mRNA in hepatocytes ± FZHY, *p<0.05, **p<0.01. (C) The levels of ALT were measured in the supernatants of CCl₄-treated hepatocytes ± FZHY (0.08 or 1.25 μg/μl), *p<0.05, **p<0.01. The data is average of at least three independent experiments (C). FZHY recipe suppresses CCl₄-induced apoptosis of hepatocytes. The effect of FZHY on CCl₄-induced apoptosis of hepatocytes was evaluated by co-staining of cultured hepatocytes with Propidium Iodide (PI, to detect nuclei of apoptotic cells) and Hoechst (to visualize the total cell nuclei). The representative images of hepatocytes ± FZHY (0.08 or 1.25 μg/μl) are shown using objective x20.