Figure 4:
FZHY compound suppresses hepatic lipid peroxidation and ROS production via inhibition of NOX expression in hepatocytes.
Liver tissues from Oil- or CCl4-injured mice ± FZHY (4.0g/kg body weight, n=10 per group); and from sham- or BDL-operated mice ± FZHY (4.0g/kg body weight, n=10 per group) were analyzed for the presence of lipid peroxidation and NOX expression. (A) Immunofluorescence staining of 4-HNE (green, ×20), representative images are shown. (B) MDA levels were measured in liver tissues using TBARS assay, the results are average of three independent experiments, and presented as nmol/mg protein, p<0.05, **p<0.01. (C) Expression of NOX4 was measured by RT-PCR in livers of Oil- or CCl4-injured mice ± FZHY, (D) and sham- or BDL-operated mice ± FZHY, *p<0.05, **p<0.01. Expression of NOX1 and NOX2 in these mice, but was not changed upon FZHY administration (data not shown).