Figure 5:

FZHY recipe does not inhibit HSC activation in vitro.

qHSCs were isolated from Collagen-α1(I)-GFP mice and plastic activated. (A) GFP fluorescence (green, ×20) of HSCs incubated ± FZHY (60ug/ml). The same number of GFP⁺ cells were detected in HSCs cultured HSCs incubated ± FZHY, indicating that FZHY has minimal effect on HSC activation. Representative images are shown using objective x20. (B) Expression of fibrogenic cytokines including collagen-α1(I), α-SMA, TNF-β1and TGFRI in HSCs incubated ± FZHY (60ug/ml), *p<0.05, **p<0.01