FZHY inhibits recruitment and activation of inflammatory macrophages in fibrotic liver.
Liver tissues from Oil- or CCl4-injured mice ± FZHY (4.0g/kg body weight, n=10 per group); and from sham- or BDL-operated mice ± FZHY (4.0g/kg body weight, n=10 per group) were analyzed for the expression of myeloid cell markers and inflammatory and fibrogenic cytokines. (A) Immunofluorescent staining for F4/80 (green, ×20), representative images are shown. (B) Positive area for F4/80 staining is calculated as percent, *p<0.05, **p<0.01. (C) Expression of myeloid genes and pro-inflammatory mediators was measured by RT-PCR in liver tissues from Oil- CCl4-injured mice ± FZHY and (D) sham- or BDL-operated mice ± FZHY, *p<0.05.