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. 2020 Nov 9;2020:8867349. doi: 10.1155/2020/8867349

Figure 1.

Figure 1

Characterization of iPSCs, embryoid body, and teratoma. (a) Counterstaining the nucleus with DAPI as blue color. Immunofluorescence staining against pluripotency markers including NANOG (red), OCT4 (green), and SOX2 (pink). The last figure was a merged picture. Scalebars = 50μm. (b) Counterstaining the nucleus with DAPI as blue color. Immunofluorescence staining of embryoid body differentiation against TUJ-1 (ectoderm, green in color), BRACHYURY (mesoderm, red in color), and AFP (endoderm, pink in color). (c) RT-PCR of pluripotency gene expressions of iPSCs. The iPSCs were positive for pluripotency markers including OCT4, SOX2, and NANOG. (d) RT-PCR of gene expression of iPSC-derived teratoma. The teratoma expressed genes of three germ layers, including GATA4 (endoderm), HAND1, GATA6 (mesoderm), TUBB3, MAP2, and GFAP (ectoderm).