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. 2020 Nov 9;2020:8867349. doi: 10.1155/2020/8867349

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Copyright © 2020 Yu-Hsun Chang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The process from iPSC to mesenchymal stem cell (MSC). (a) The fibroblastic morphology cells (Lt side of the figure) appeared on day 7 after differentiation around the epithelial round cell colony (Rt side of the figure). (b) At day 14, more fibroblast-like cells appeared. (c) At day 21, around 100% of cells were fibroblastic appearance cells. (d) On day 29, fully differentiated MSC were noted. Scalebar = 1000μm. (e) qPCR showed a decreasing trend of pluripotency gene (OCT4) expression from postdifferentiation day 7 to day 29. (f) qPCR showed an increasing trend of MSC gene (CD73) expression from postdifferentiation day 7 to day 29. ^∗∗∗p < 0.001. We used an ANOVA test with a post hoc analysis with the Bonferroni test.