Table 1.
Gene primer sequences used for RT-PCR and qPCR experiments.
| Gene name | Forward sequence | Reverse sequence | Base pair no. |
|---|---|---|---|
| Oct4 | CAG TGC CCG AAA CCC ACA C | CAG TGC CCG AAA CCC ACA C | 161 |
| Nanog | AGT CCC AAA GGC AAA CAA CCC ACT TC | TGC TGG AGG CTG AGG TAT TTC TGT CTC | 161 |
| Sox2 | GGG AAA TGG GAG GGG TGC AAA AGA GG | TTG CGT GAG TGT GGA TGG GAT TGG TG | 151 |
| MAP2 | GCA TGA GCT CTT GGC AGG | CCA ATT GAA CCC ATG TAA AGC C | 194 |
| GFAP | AGG GCT GAC ACG TCC AC | GCC TTA GAG GGG AGA GGA G | 132 |
| GATA4 | TCC CTC TTC CCT CCT CAA AT | TCA GCG TGT AAA GGC ATC TG | 194 |
| Tubb3 | CAG AGC AAG AAC AGC AGC TAC TT | GTG AAC TCC ATC TCG TCC ATG CCC TC | 227 |
| Hand1 | TGC CTG AGA AAG AGA ACC AG | ATG GCA GGA TGA ACA AAC AC | 274 |
| GATA6 | CCT CAC TCC ACT CGT GTC TGC | GTC CTG GCT TCT GGA AGT GG | 225 |
| PPARr | AGC CTC ATG AAG AGC CTT CCA | TCC GGA AGA AAC CCT TGC A | 120 |
| OPN | AGG AGG AGG CAG AGC ACA | CTG GTA TGG CAC AGG TGA TG | 150 |
| APAL | CCACGTCTTCACATTTGGTG | GCAGTGAAGGGCTTCTTGTC | 96 |
| FABP4 | ATGGGATGGAAAATCAACCA | GTGGAAGTGACGCCTTTCAT | 87 |
| Aggrecan | CGAAACATCACTGAGGGTGA | GCAAACGTGAAGGGCTCCT | 107 |
| Col2A1 | GAGAGGTCTTCCTGGCAAAG | AAGTCCCTGGAAGCCAGAT | 118 |
| CD73 | AGTCCACTGGAGAGTTCCTGCA | TGAGAGGGTCATAACTGGGCAC | 133 |
| OCT4 | CTT GCT GCA GAA GTG GGT GGA GGA | CTG CAG TGT GGG TTT CGG GCA | 169 |
| GAPDH | GGTCTCCTCTGACTTGAACA | GTGAGGGTCTCTCTCTTCCT | 221 |
| KCa1.1 | ACAACATCTCCCCCAACC | TCATCACCTTCTTTCCAATTC | 310 |
| KCa3.1 | CGGGAACAAGTGAACTCCAT | ACTGGGGAAAGTAGCCTGGT | 239 |
| Kv1.2 | ATGAGAGAATTGGGCCTCCT | CCCACTATCTTTCCCCCAAT | 200 |
| Kv1.4 | ACGAGGGCTTTGTGAGAGAA | CACGATGAAGAAGGGGTCAT | 308 |
| Kv1.5 | GTAACGTCAAGGCCAAGAGC | GGGAGGAAAGGAGTGAAAGG | 225 |
| Kv1.6 | CTGGCTTGACCACAGTCTGA | CTGGAGTTTGCCTGAGGAAG | 190 |
| Kv2.1 | GTTGGCCATTCTGCCATACT | GCAAAGTGAAGCCCAGAGAC | 173 |
| Kv4.2 | GCTTGTCATCAATCCCCTTG | TCCAGTATCTGGGCTTTTCC | 102 |
| Kv4.3 | ACGGAGACATGGTGCCTAAG | CCCTGCGTTTATCAGCTCTC | 153 |
| KCNH1 | TGGATTTTGCAAGCTGTCTG | GAGTCTTTGGTGCCTCTTGC | 476 |
| Kir2.1 | AACAGGGAGGTGTGGACAAG | TAACCTGCTCTAGGGCTCCA | 261 |
| Kir2.2 | GAGGCTATCACAGGCTCAGG | CCCCAAGTTAAAAACCAGCA | 183 |
| Kir2.3 | GCTTTGAGCCTGTGGTCTTC | TTGGCTCTGTCCTGAGTGTG | 480 |
| Clcn3 | CATAGGTCAAGCAGAGGGTC | TATTTCCGCAGCAACAGG | 293 |
| SCN9A | GCTCCGAGTCTTCAAGTTGG | GGTTGTTTGCATCAGGGTCT | 446 |
| CACNA1C | AACATCAACAACGCCAACAA | AGGGCAGGACTGTCTTCTGA | 574 |
| GAPDH | CCATCTTCCAGGAGCGAG | GCAGGAGGCATTGCTGAT | 233 |
| rCollagen 2a | CCTGTGCGACGACATAATCTGT | GGTCCTTTAGGTCCTACGATATCCT | 176 |
| rCOL1 | CAGAACGGCCTCAGGTACCA | CAGATCACGTCATCGCACAAC | 101 |
| rTNFa | CTGCACTTCAGGGTGATCG | CTACGTGGGCTAGAGGCTTG | 133 |
| rIL1B | TTGAAGAAGAACCCGTCCTCTG | CTCATACGTGCCAGACAACACC | 128 |
| rMMP13 | AGGAAGACCTCCAGTTTGCAGAG | GCTGCATTCTCCTTCAGGATTC | 85 |
| rGAPDH | TGACGACATCAAGAAGGTGGTG | GAAGGTGGAGGAGTGGGTGTC | 120 |