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. 2020 Nov 16;88(12):e00363-20. doi: 10.1128/IAI.00363-20

FIG 1.

FIG 1

Pseudomonas aeruginosa infection-impaired NK cell cytotoxicity in response to B221 cell line. NK cell cytotoxic activity (CD107a) among PBMCs was assessed with or without infection and with (+) or without (−) B221 cell exposition (see Fig. SA1 in the supplemental material). (A) Representative density plots illustrating CD107a expression in NK cells (PerCp-CD3− APC-CD56+ eFluor780− in lymphocyte gate) by flow cytometry. Histograms illustrating CD107a+ (B) and the mortality rate of NK cells (C) in noninfected (NI) or 24-h Pseudomonas aeruginosa-infected (PA) conditions. Data are shown as the median and interquartile range of 10 distinct healthy donors. *, P < 0.05; NS, nonsignificant difference; B221, 5-h exposition of PBMC to B221 cells with a B221/PBMC ratio of 1:1; APC-H7 eFluor 780, viability assessment.