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. 2020 Nov 16;88(12):e00363-20. doi: 10.1128/IAI.00363-20

FIG 2.

FIG 2

NKG2D-activating receptor is involved in NK cell cytotoxic response against B221 cells. (A) Histogram profiles for different ligands of activating NK cell receptors expressed on B221 cells as follows: ULPB1 to ULPB6 and MICA/B (ligands of NKG2D), B7-H6 (ligand of NKp30), CD48 (ligand of 2B4), and CD112 and CD155 (ligands of DNAM-1). Isotype-matched negative controls are shown as gray-filled curves and ligands as white-filled curves. (B, C, and D) 2B4, NKp30, and NKG2D mean fluorescence intensity (MFI) on NK cells in noninfected or 24-h Pseudomonas aeruginosa-infected (PA) PBMCs (without B221 cell exposition). (E) Representative histograms of CD107a activity in sorted NK cells in response to B221 cells with or without anti-NKG2D blocking antibody or its isotype. Data are shown as the median and interquartile range of 6 (B, C, and D) and 4 (E) distinct healthy donors. NK cells were analyzed in lymphocyte gate by flow cytometry after PerCp-CD3− APC-CD56+ eFluor780− staining. *, P < 0.05; NS, nonsignificant difference; B221, 5-h exposition to B221 cells with a B221/NK ratio of 10:1 in order to keep the same ratio as in PBMCs (assuming 10% of NK cells in PBMC).