Skip to main content
. 2020 Nov 16;88(12):e00545-20. doi: 10.1128/IAI.00545-20

FIG 4.

FIG 4

F-actin, host cell microtubule dynamics, and vesicular trafficking do not play a role in the localization of aldolase A at the inclusion. HeLa cells expressing 3×FLAG-AldoA and infected with the mCherry CtL2 strain were fixed at 24 h postinfection and immunostained with anti-FLAG antibodies. (A, D, and E) Quantification of the percentage of inclusions positive for 3×FLAG-AldoA following treatment with 1 μM cytochalasin D (Cyto D) at 23.5 h postinfection (A), 33 μM nocodazole at 23.5 h postinfection (D), or 1 μg/ml brefeldin A at 6 h postinfection (E). (B) Quantification of the percentage of inclusions positive for 3×FLAG- AldoA in HeLa cells expressing wild-type 3×FLAG-AldoA (WT), or point mutants no longer able to bind actin 3×FLAG-R42A AldoA (R42A) and 3×FLAG-R148A AldoA (R148A). (C) Quantification of the percentage of inclusions positive for 3×FLAG-AldoA in HeLa cells infected with wild-type C. trachomatis (WT) or an inaC mutant strain (inaC::aadA). Data show the means and the SD of a combination of three independent experiments. ns, not statistically significant (Student t test).