Figure 2.

In vivo hACE2 pulmonary transfection in mice

Mice underwent pulmonary transfection with hACE2 expression plasmid (35 μg each) or control pCMV3 plasmid via orotracheal intubation, after which lungs were harvested at different days post-transfection. A: Expression of hACE2 in transfected lung tissue via western blotting. B: Lung sections at 2 days post-transfection were stained with anti-hACE2 antibody and hematoxylin (original magnification, 40×). Red arrows indicate obvious expression of hACE2. C: Immunofluorescence detection of proSP-C and hACE2 proteins in lung tissue sections at 2 days post-transfection. D: Lung sections at 2 and 7 days post-transfection were stained with hematoxylin and eosin for histopathological analysis (original magnification, 20×).