Table 2.
Hemodynamic analysis of inducible cardiomyocyte-specific Trpm4flox/flox; αMHC-CreERT2 mice. Creneg, n = 4; Crepos, n = 6
| C57Bl/6N | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Isoproterenol (ng/kg/min) | 0 | 9 | 30 | 60 | 90 | 300 | ||||||
| Genotype: Trpm4flox/flox | Cre- | Cre + | Cre- | Cre + | Cre- | Cre + | Cre- | Cre + | Cre- | Cre + | Cre- | Cre + |
| HR (b/min) | 479 ± 16 | 478 ± 12 | 487 ± 18 | 484 ± 13 | 559 ± 10 | 548 ± 15 | 638 ± 15 | 641 ± 12 | 677 ± 17 | 694 ± 5 | 696 ± 9 | 707 ± 5 |
| SV (µl) | 9.6 ± 0.4 | 9.3 ± 1.0 | 10.2 ± 0.5 | 9.4 ± 1.0 | 12.0 ± 0.9 | 11.3 ± 0.8 | 14.7 ± 1.2 | 13.3 ± 0.8 | 15.5 ± 1.7 | 14.3 ± 1.0 | 15.9 ± 1.5 | 14.6 ± 1.1 |
| CO (µl/min) | 4604 ± 214 | 4392 ± 450 | 4967 ± 312 | 4515 ± 445 | 6704 ± 451 | 6190 ± 491 | 9325 ± 641 | 8529 ± 406 | 10,460 ± 1071 | 9943 ± 635 | 11,075 ± 1083 | 10,298 ± 822 |
| Ved (µl) | 26.6 ± 3.5 | 23.2 ± 2.5 | 25.9 ± 3.2 | 22.9 ± 2.4 | 22.0 ± 2.7 | 18.6 ± 2.0 | 17.9 ± 2.1 | 15.1 ± 1.7 | 16.9 ± 2.1 | 14.0 ± 1.8 | 16.8 ± 2.0 | 14.5 ± 1.8 |
| Ves (µl) | 18.3 ± 3.7 | 15 ± 9 | 17.6 ± 3.3 | 15.5 ± 2.4 | 11.7 ± 2.3 | 9.5 ± 2.3 | 4.0 ± 1.2 | 2.6 ± 1.7 | 2.2 ± 1.0 | 1.4 ± 1.0 | 2.6 ± 1.3 | 1.6 ± 1.2 |
| Ped (mmHg) | 3.7 ± 1.0 | 2.7 ± 0.8 | 3.5 ± 0.8 | 2.8 ± 0.7 | 4.3 ± 2.1 | 2.1 ± 0.7 | 2.8 ± 0.7 | 1.9 ± 0.8 | 2.8 ± 0.6 | 1.4 ± 1.5 | 4.2 ± 1.9 | 2.5 ± 1.1 |
| Systolic parameters | ||||||||||||
| SW (mmHg*s) | 489 ± 7 | 464 ± 79 | 511 ± 12 | 463 ± 66 | 689 ± 68 | 585 ± 67 | 864 ± 102 | 717 ± 59 | 892 ± 93 | 812 ± 75 | 937 ± 99 | 862 ± 98 |
| dP/dtmax (mmHg/s) | 393 ± 229 | 3711 ± 196 | 4113 ± 316 | 3800 ± 160 | 5648 ± 361 | 4862 ± 394 | 8205 ± 964 | 6768 ± 234 | 9733 ± 465 | 9015 ± 280 | 11,763 ± 1303 | 10,241 ± 449 |
| PRSW (mmHg) | 65.0 ± 0.9 | 58.0 ± 0.9 | 62.6 ± 1.4 | 62.3 ± 1.2 | 75.1 ± 1.0 | 75.5 ± 1.0 | 8.0 ± 0.8 | 79.4 ± 1.0 | 78.9 ± 0.7 | 83.2 ± 0.5 | 77.1 ± 0.6 | 83.5 ± 0.2 |
| Diastolic parameters | ||||||||||||
| -dP/dtmin (mmHg/s) | – 4827 ± 417 | – 4467 ± 211 | – 5012 ± 571 | – 4686 ± 228 | – 6657 ± 1048 | – 5492 ± 365 | – 6643 ± 587 | – 5994 ± 541 | – 5969 ± 532 | – 6289 ± 569 | – 7793 ± 1491 | – 6488 ± 526 |
| Tau (ms) | 8.3 ± 0.6 | 8.0 ± 0.4 | 7.7 ± 0.4 | 7.7 ± 0.4 | 5.7 ± 0.3 | 5.8 ± 0.3 | 4.9 ± 0.6 | 4.6 ± 0.2 | 4.7 ± 0.5 | 4.5 ± 0.3 | 4.6 ± 0.4 | 4.6 ± 0.2 |
HR, heart rate; SV, stroke volume; CO, cardiac output; Ved, end-diastolic volume; Ves, end-systolic volume; Ped, end-diastolic pressure; SW, stroke work; dP/dtmax, peak rate of pressure rise; -dP/dtmin, peak rate of pressure decline; PRSW, preload recruited stroke work; Tau, relaxation time constant (Weiss method). Values are expressed as means ± SEM