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. 2020 Nov 4;11:591019. doi: 10.3389/fmicb.2020.591019

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Copyright © 2020 Ziraldo, Bidart, Prato, Tribulatti, Zamorano, Mattion and D’Antuono.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assembly of recombinant capsid proteins into subviral particles. (A) Lysates of HEK 293A cells infected with Ad5[P], Ad5[P]_OP, or Ad5[P_VP2]_OP were loaded onto 10–45% sucrose gradients. Inactivated native A/Argentina/2001 foot-and-mouth disease virus (iFMDV A/Arg/01) was also included as a marker (black dotted line). Fractions were collected after centrifugation at high speed and analyzed by ELISA. The positions of the FMDV virions (146S), empty capsids (75S), and capsomers (12S) are indicated. (B) Eight fractions (5, 10, 15, 20, 25, 30, 35, and 40) were analyzed by Western blot with specific anti-FMDV O1/Campos polyclonal antibody. VP0 and VP1/VP3 are indicated with an arrow. (C) Negative-staining TEM of in situ generated FMDV empty capsid particles (right) and iFMDV 146S and 75S particles (left). Scale bar, 50 nm. Virus-like particles are indicated (filled inverted triangle).