FIGURE 7.

Protection against challenge with infectious foot-and-mouth disease virus (FMDV) O1/Campos. (A) Protection from viral challenge elicited by the different Ad5-FMD vectors. Groups of mice were inoculated with Ad5[P], Ad5[P]_OP, Ad5[P_VP2]_OP, Ad5[G], or a commercial FMD vaccine and challenged with infective FMDV at 49 dpi. Animals were considered protected if viremia was absent after a blind passage. Protection percentages were calculated as 100 × (number of vaccinated animals without viremia/number of vaccinated animals). (B) Virus neutralization test (VNT) at day 45 post-inoculation with 5 × 10⁸ PFU, expressed as log₁₀ of the reciprocal of the serum dilution that neutralizes 50% of 100 TCID₅₀ of infective FMDV O1/Campos (fixed virus–variable serum method). *p < 0.05, **p < 0.01 with respect to the Ad5[P] group. A serum pool was made every two animals of a group in order to perform the serum neutralization test.