FIGURE 8.

Bone marrow dendritic cell (BMDC) activation induced by Ad5[P] or Ad5[P_VP2]_OP infection. BMDCs were infected with Ad5[P] or Ad5[P_VP2]_OP at multiplicity of infection (MOI) of 50 and 250, as described in the section “Materials and Methods.” Then, the cells were stained with specific monoclonal antibodies (mAbs) for phenotypic and activation markers and analyzed by flow cytometry. (A) Representative histograms depict the fluorescence intensities of CD80, CD86, and major histocompatibility complex (MHC) class II molecules on MHCII⁺CD11c⁺ gated population. (B) Bars represent the geometric mean of fluorescence intensity (MFI) ± SD. Isotype, cells were stained with control isotype mAbs; LPS-stimulated, cells were treated with 500 ng/ml lipopolysaccharide (LPS). *p < 0.05, **p < 0.01, ***p < 0.001 compared to non-stimulated cells (ANOVA).