TABLE 1.

qPCR and qPCDR amplification of SARS-CoV-2 cDNA.

SARS-CoV-2 cDNA copies per reaction	5000	500	50	5	NTC
Cq of qPCR	29.61 ± 0.04	33.25 ± 0.07	35.94 ± 0.04	NA	NA
Cq of qPCDR	22.72 ± 0.06	28.09 ± 0.05	33.20 ± 0.09	37.66 ± 1.36	NA
ΔCq	6.89	5.16	2.74	–	–

The mean quantification cycles (Cq) ± standard deviation of three replicates for the indicated cDNA copy numbers are provided. The amplifications were carried out with SD HotStart DNA polymerase using a series of SARS-CoV-2 cDNA templates with 10-fold dilutions from 5000 to 5 copies per reaction (NTC, no template control). ΔCq is the difference between the Cq of the qPCR and qPCDR for each template dilution.