TABLE 2.

Comparison of RT-qLAMP and RT-q(PCR-LAMP) assays.

SARS-CoV-2 RNA copies per reaction	5000	500	50	5	NTC
Tq (min) of qLAMP	27.51 ± 0.24	31.47 ± 3.72	NA	NA	NA
Tq (min) of q(PCR-LAMP); 1 cycle of PCR	21.30 ± 0.98	23.44 ± 4.27	28.44 ± 5.03	30.40 ± 3.39	NA
Tq (min) of q(PCR-LAMP); 2 cycles of PCR	19.88 ± 0.85	21.69 ± 1.72	25.83 ± 5.44	26.86 ± 6.17	NA
Tq (min) of q(PCR-LAMP); 4 cycles of PCR	18.10 ± 0.29	20.70 ± 0.89	23.31 ± 4.18	26.24 ± 5.06	NA
Tq (min) of q(PCR-LAMP); 6 cycles of PCR	15.56 ± 0.90	19.54 ± 0.67	20.81 ± 0.76	22.23 ± 0.93	NA

SARS-CoV-2 RNA was detected by RT-qLAMP and RT-q(PCR-LAMP) assays. The q(PCR-LAMP) amplifications were performed with 1, 2, 4, and 6 cycles of PCR. The table lists the mean quantification times (Tq) ± standard deviation of three replicates of the qLAMP and q(PCR-LAMP) reactions for the indicated RNA copy numbers. The assays were carried out with Reverase reverse-transcriptase and SD DNA polymerase using a series of SARS-CoV-2 RNA 10-fold dilutions from 5000 to 5 copies per reaction (NTC, no template control).