Figure 2.

In vitro modulation of RBC deformability, eryptosis, and RBC-MPs by oxidative stress and NO related agents on SCA RBCs. Impact of NAC (n = 7) on SCA RBC deformability (A), RBC PS exposure (B), ROS level (C), RBC Ca²⁺ content (D) and glucose uptake (E). Effect of cumene hydroperoxide (n = 7) on SCA RBC deformability (F), RBC PS exposure (G), ROS level (H), RBC Ca²⁺ content (I) and glucose uptake (J). Effect of SNP (n = 7) on SCA RBC deformability (K), RBC PS exposure (L), ROS level (M), RBC Ca²⁺ content (N) and glucose uptake (O). Impact of NAC, cumene hydroperoxide, SNP and L-NIO on the release of MPs by RBCs in the supernatant (P) (n = 7). Significantly different from the control condition: *p < 0.05, **p < 0.01. ns, not significant. All statistical comparisons were performed using paired T-test except for the MPs modulation where a Friedmann test was used. MFI, mean fluorescence intensity; %RBC PS+, percentage of RBCs positive to Annexin-V; ROS, level of reactive oxygen species in RBCs detected with DCFDA; Ca2+, level of calcium in RBCs detected with Fluo3; NAC: N-Acetylcysteine; SNP, sodium nitroprusside; Cumene, cumene hydroperoxide; −, incubation with ctrl vehicle, +, incubation with pharmacological molecule.