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. 2020 Nov 17;10:20003. doi: 10.1038/s41598-020-77103-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Aspect ratio of MC3T3-E1 cells on gratings with different depths. Cell shape was observed at 0, 4, 8, 12, and 16 h and the mean aspect ratio was calculated. One-way ANOVA and Tukey’s post hoc test with **p < 0.01 and ***p < 0.001. Micrographs represent MC3T3-E1 cell elongation on different gratings.