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. 2020 Nov 17;10:19954. doi: 10.1038/s41598-020-75814-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematics of the simulator and experimental system. (a) General principle of FluoSim. (b) Contact between two COS cells, one expressing GFP-Nrx1β (green) and the other expressing Nlg1-mCherry (magenta), resulting in molecule accumulation through adhesive interactions (yellow zone). (c) Diagram showing a zoomed section of the cell–cell interface at the coverslip; the yellow beam represents oblique laser illumination. (d) Cartoon of Nrx1β diffusional trapping by Nlg1 in a cell–cell contact.