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. 2020 Nov 17;10:19954. doi: 10.1038/s41598-020-75814-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fitting SRI experiments. (a) Representative single frame image of a STORM sequence performed on GFP-Nrx1β labeled with Alexa647-conjugated GFP nanobody, and corresponding super-resolved image generated from 3.57 × 10⁶ single molecule localizations (pixel size 32 nm, total acquisition time 1600 s). The number of molecules detected per pixel is color coded. (b) Simulated image showing single molecule fluorescence emission in the same cell geometry, and corresponding super-resolved map with a localization precision of 58 nm (FWHM). The total number of detections is 3.61 × 10⁶. (c) Fluorescence emission over time from an isolated Alexa647-conjugated GFP nanobody bound to the glass coverslip in a STORM sequence, and simulated emission of fluorescence of an immobile molecule obtained with switch-on and -off rates of 0.006 s⁻¹ and 9.3 s⁻¹, respectively.