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. 2020 Nov 17;10:19936. doi: 10.1038/s41598-020-76271-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

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Effect of Pf on neutrophil cell health. Media alone (mock) or 20 ng/ml of LPS (LPS)-activated neutrophils were treated with 0 or 0.5 mg/ml Pf (+ Pf) along with (b) Cytotox-red or (a, c) Annexin V-red and Caspase-3/7-green to measure toxicity or apoptosis respectively by live cell fluorescent imaging of events using Incucyte S3 system. H₂O₂- and Ssp (staurosporine)-treated neutrophils were used as positive controls for measuring necrosis/toxicity and apoptosis respectively. Representative individual images with neutrophils double stained with AnnexinV—red and caspase3/7—green along with overlay-image is shown (a). Data points in the graphs show the percentage of positive dead cells (b) and apoptotic cell percentage (b) calculated from mean ± SEM of 6 different experiments done in triplicates. *Represents significant difference (p-value ≤ 0.05; two-way ANOVA) between various treatment groups.