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. 2020 Nov 17;10:19936. doi: 10.1038/s41598-020-76271-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pf reduces chemotactic migration of neutrophils. Mock or LPS-activated neutrophils were treated with ± 0.5 mg/ml Pf and their migration towards chemo-attractants fMLP (300 nM), LTB4 (100 nM) and IL-8 (100 ng/ml) were measured in a trans-well assay system by Incucyte S3 live cell imaging system. Representative images with neutrophils in top well and migrated cells in bottom trans-well and efficiency of mask in identifying migrated cells (red arrow pointing migrated cells) is shown (a). Cytochalasin-D (cytD)-treated neutrophils were used as a negative control for neutrophil chemotaxis. The graphs represent the time course of migrated neutrophil number in response to chemotactic agents fMLP (b), LTB4 (c), IL-8 (d). Data points were calculated from mean ± SEM obtained from 6 different experiments done in triplicate. *Represents significant difference (p-value ≤ 0.05; two-way ANOVA) between various treatment groups.