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. 2020 Nov 17;10:19936. doi: 10.1038/s41598-020-76271-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pf decreases neutrophil degranulation. Mock or LPS-activated neutrophils were treated with ± 0.5 mg/ml Pf and incubated for 4 h. Cells and supernatants were collected and analyzed for degranulation markers and proteins. Cells were stained for degranulation markers using fluorescent antibodies and quantified by flow cytometry for % cells (a) and MFI (b). Supernatants were analyzed for MPO and quantity of degranulation proteins (MMP8, MMP9, and NGAL) by fluorescent MPO assay and Procarta-Multiplex Immunoassay respectively (c). Data points in the graphs represent mean ± SEM values obtained from 6 different experiments done in triplicate. *Represents significant difference (p-value ≤ 0.05; two-way ANOVA) between various treatment groups.