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. 2020 Nov 17;11:5845. doi: 10.1038/s41467-020-19624-w

Fig. 2. Translocation of MoHTR1 and MoHTR2 into rice cell and migration into rice nuclei.

Fig. 2

The MoHTR1:mRFP and MoHTR2:mRFP expressed under control of their native promoter were observed at an early infection stage (30 hpi). BAS4sp:eGFP is a marker for intact extra-invasive hyphal membrane. a MoHTR1:mRFP and b MoHTR2:mRFP were mainly localized in BICs and the nuclei of invaded rice leaf sheath cells. The images shown were chosen from confocal optical z-sections taken at 1-μm intervals. At 48 hpi, c MoHTR1:mRFP and d MoHTR2:mRFP were also detected in the nuclei of some uninfected cells. Arrowheads indicate BICs, and arrows indicate rice nuclei. Asterisks indicate rice cells invaded by fungal hyphae. Scale bars = 10 μm. Representative micrographs are shown from three independently infected rice sheaths and additional representative data are provided as a Source Data file.