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. 2020 Nov 17;10:19997. doi: 10.1038/s41598-020-77095-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bio-distribution of TRAIL, TRAIL-ATNC, and TRAIL-ATNC^IL4rP. (A) Representative images of the bio-distribution of TRAIL, TRAIL-ATNC, and TRAIL-ATNC^IL4rP. Mice bearing MDA-MB-231 tumor were intravenously injected with FPI774-labeled TRAIL, TRAIL-ATNC, or TRAIL-ATNC^IL4rP followed by in vivo scanning by IVIS imaging system. Equal amounts of fluorescence of proteins were injected. (B,C) At 24 h post-injection, mice were sacrificed and organ/tissues were collected. Ex vivo bio-distribution was examined for TRAIL, TRAIL-ATNC, and TRAIL-ATNC^IL4rP mice group. The average fluorescence intensity of each organ was measured (C). Data represent means ± SEM (**p < 0.01; T-test).