Figure 1.

The mucin-like domain was poorly immunogenic for ChAd3-EBOZ volunteers. T-cell Elispot performed on sera from volunteers of the Lausanne clinical trial, whose T-cells were stimulated with a pool of 15-mers overlapping peptides covering the entire sequence of the GP protein [(A) left graph] or the same pool without the region corresponding to the mucin-like domain [(A), right graph]. Analysis was performed before vaccination (D0) or 28 days after vaccination (D28) in the placebo group, as well as in the groups of volunteers immunized with the ChAd3-EBOZ vaccine at low dose or high dose. In (B) the contribution of each pool to T-cells stimulation is highlighted. Information about the GP region covered by each pool are reported in the figure. The size of the pies correlates with intensity.