Figure 3.

The insertion of a C-terminal trimerization motif favors the formation of the α-helical bundle and enhances the thermal stability of the recombinant GP. Temperature dependent CD spectra of GP ΔTM, GP ΔTM-ΔMUC, GP ΔTM-ΔMUC-T4, and GP ΔTM-ΔMUC-GCN4. Spectra were registered between 190 and 250 nm and between 20°C and 90°C at 5°C interval. For clarity, only spectra at 20°C, 75°C, and 90°C are shown. The arrows indicate the double minima at 208 and 222 nm, spectral signature typical of an α-helical secondary structure.