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. 2020 Nov 4;11:586595. doi: 10.3389/fimmu.2020.586595

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Copyright © 2020 Agnolon, Kiseljak, Wurm, Wurm, Foissard, Gallais, Wehrle, Muñoz-Fontela, Bellanger, Correia, Corradin and Spertini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant GP trimers show an increased breadth of reactivity with a panel of conformational mAbs. Sandwich ELISA analysis of GP ΔTM, GP ΔTM-ΔMUC, GP ΔTM-ΔMUC-T4, and GP ΔTM-ΔMUC-GCN4. The graphs show the results of the sandwich ELISA with human KZ52 (left) or a panel of mouse mAbs (right) as detection reagents. Average blank values were subtracted from sample values. Column heights represent the mean values of 3 independent assays, with corresponding standard deviations.