Skip to main content
. 2020 Nov 4;11:554941. doi: 10.3389/fendo.2020.554941

Figure 1.

Figure 1

Effects of isoflavones on two-dimensional (2D) or three-dimensional (3D) cell migration. (A) Chemicals structures of isoflavones and E2. (B) Representative photomicrographs showing the effects of isoflavones on 2D wound healing assays using C6 cells. Live-cell staining was performed using Cellstain-Hoechst 33258 (Dojindo Molecular Technologies, Inc., Japan). (C) Quantitative analysis of the effect of isoflavones or E2 (1 – 100 nM) on cell migration measured by wound healing assay. (D) Representative photomicrographs showing the effects of isoflavone on 3D matrigel invasion assays using astrocytes. Cell nuclei were stained with DAPI. (E) Quantitative analysis of the effect of isoflavones or E2 (1 – 100 nM) on cell invasion measured by matrigel invasion assay. The total number of cells was quantified using ImageJ software (NIH). Bars represent 50 μm. Data are expressed as the mean ± SEM (n = 30 determinations) of at least three independent experiments. **** p < 0.0001, *** p < 0.001, indicates statistical significance measured using Bonferroni’s test compared with the control (−).