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. 2020 Nov 4;11:571807. doi: 10.3389/fmicb.2020.571807

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Copyright © 2020 Leastro, Freitas-Astúa, Kitajima, Pallás and Sánchez-Navarro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Redistribution of the N protein from the N-P complex to the cell periphery and plasmodesmata upon co-expression with the MP. Confocal imagens of CiLV-N and OFV-citrus Ns-, Ns-HA, Ps-, MPs- and MPs-HA transiently co-expressed in N. benthamiana leaves visualized at 48–72 h post-infiltration. The protein pair combinations performed among these constructions are: N- + P- (Aa,Ba), MP- + P- (Ab,Bb), MP- + N-HA + -nucleus marker (Ac,Bc), N- + MP-HA + -nucleus marker (Ad,Bd), and N- + P- + MP-HA (Ae,Be). Blue arrows indicate GFP signal in the cytoplasm and white arrows indicate the GFP signal into the nucleus. The numbers at the top of Ai, Ac, Aii, Bd, and Bii panels corresponds the number of nuclei visualized expressing the correspondent protein fused to GFP from 100 counted nuclei. White boxes correspond to high magnification to highlight callose deposits stained with aniline blue () along the cell periphery (Af,Bf). All images displayed are representative of at least three independent experiments. Bars correspond to 10 μm.

Inline graphic — Redistribution of the N protein from the N-P complex to the cell periphery and plasmodesmata upon co-expression with the MP. Confocal imagens of CiLV-N and OFV-citrus Ns-, Ns-HA, Ps-, MPs- and MPs-HA transiently co-expressed in N. benthamiana leaves visualized at 48–72 h post-infiltration. The protein pair combinations performed among these constructions are: N- + P- (Aa,Ba), MP- + P- (Ab,Bb), MP- + N-HA + -nucleus marker (Ac,Bc), N- + MP-HA + -nucleus marker (Ad,Bd), and N- + P- + MP-HA (Ae,Be). Blue arrows indicate GFP signal in the cytoplasm and white arrows indicate the GFP signal into the nucleus. The numbers at the top of Ai, Ac, Aii, Bd, and Bii panels corresponds the number of nuclei visualized expressing the correspondent protein fused to GFP from 100 counted nuclei. White boxes correspond to high magnification to highlight callose deposits stained with aniline blue () along the cell periphery (Af,Bf). All images displayed are representative of at least three independent experiments. Bars correspond to 10 μm.