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. 2020 Nov 4;11:571807. doi: 10.3389/fmicb.2020.571807

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Copyright © 2020 Leastro, Freitas-Astúa, Kitajima, Pallás and Sánchez-Navarro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BiFC analyses of the transient expression combinations between movement proteins (MP), nucleoproteins (N), and capsid protein (p29) from distinct BTVs. Dimer (A), intra (B), and inter (C,D) interactions of the p29 and MP of the CiLV-C2, and Ns and MPs of the CiLV-N and OFV-citrus, were analyzed by BiFC. p29, Ns and MPs carrying N-terminal () or C-terminal () YFP fragments fused at their N- (/-ORFs) or C-termini (ORFs-/) were transiently co-expressed in N. benthamiana leaves by agroinfiltration. Representative protein pair combinations are indicated at the left of each image; all hetero-combinations performed are shown in Table 3. All images contain two pictures corresponding to the YFP signal or merged with bright field. The YFP fluorescence reconstitution was monitored at 4 days post-infiltration using a confocal laser scanning microscope Zeiss LSM 780 model. Suggested positive interactions were visualized in (i,iii,iv,vi–viii,ix,xi–xiii,xiv). No interactions are indicated in (ii,v,x,xv,xvi). Negative controls correspond to the expression of the respective proteins in combination with Cyt or ER BiFC vectors (E). Additional negative controls are presented for the intra-association: CiLV-C MP + p24 (putative matrix protein) (Leastro et al., 2018) and for the inter-association between genera: AMV CP + CiLV-C MP and TSWV N + CiLV-C MP (Leastro et al., unpublished) (E). All images displayed are representative of three independent experiments. Bars correspond to 50 μm.

Inline graphic — BiFC analyses of the transient expression combinations between movement proteins (MP), nucleoproteins (N), and capsid protein (p29) from distinct BTVs. Dimer (A), intra (B), and inter (C,D) interactions of the p29 and MP of the CiLV-C2, and Ns and MPs of the CiLV-N and OFV-citrus, were analyzed by BiFC. p29, Ns and MPs carrying N-terminal () or C-terminal () YFP fragments fused at their N- (/-ORFs) or C-termini (ORFs-/) were transiently co-expressed in N. benthamiana leaves by agroinfiltration. Representative protein pair combinations are indicated at the left of each image; all hetero-combinations performed are shown in Table 3. All images contain two pictures corresponding to the YFP signal or merged with bright field. The YFP fluorescence reconstitution was monitored at 4 days post-infiltration using a confocal laser scanning microscope Zeiss LSM 780 model. Suggested positive interactions were visualized in (i,iii,iv,vi–viii,ix,xi–xiii,xiv). No interactions are indicated in (ii,v,x,xv,xvi). Negative controls correspond to the expression of the respective proteins in combination with Cyt or ER BiFC vectors (E). Additional negative controls are presented for the intra-association: CiLV-C MP + p24 (putative matrix protein) (Leastro et al., 2018) and for the inter-association between genera: AMV CP + CiLV-C MP and TSWV N + CiLV-C MP (Leastro et al., unpublished) (E). All images displayed are representative of three independent experiments. Bars correspond to 50 μm.