TABLE 1.
Protein pair combinations performed in the BiFC topology assay for MPs and Ns proteins of the CiLV-N and OFV-citrus.
| Membrane topology | NYFP-Cyt | CYFP-Cyt | NYFP-ER | CYFP-ER | |
| Control | NYFP-ER | − | + | ||
| NYFP-Cyt | + | − | |||
| CiLV-N | N-NYFP | + | − | ||
| N-CtYFP | − | − | |||
| NYFP-N | + | − | |||
| CYFP-N | − | − | |||
| MP-NYFP | + | − | |||
| MP-CYFP | − | − | |||
| NYFP-MP | + | − | |||
| CYFP-MP | − | − | |||
| OFV-citrus | N-NYFP | + | − | ||
| N-CYFP | − | − | |||
| NYFP-N | + | − | |||
| CYFP-N | − | − | |||
| MP-NYFP | + | − | |||
| MP-CYFP | − | − | |||
| NYFP-MP | + | − | |||
| CYFP-MP | − | − | |||
The symbols − and + correspond to the absence and presence of fluorescence signals, respectively. NYFP and CYFP correspond to half N- and C-terminal of the YFP protein, respectively.