TABLE 3.
Protein pair combinations performed in the BiFC heterologous assays.
| Heterologous assays | p29/MP CiLV-C2 | N/MP CiLV-N | P/N CiLV-N | P/MP CiLV-N | N/MP OFV |
| ORF-NYFP + ORF-CYFP | + | + | − | + | − |
| ORF-NYFP + CYFP-ORF | − | − | + | + | − |
| NYFP-ORF + ORF-CYFP | − | − | − | + | − |
| NYFP-ORF + CYFP-ORF | − | − | + | + | + |
| ORF-CYFP + ORF-NYFP | + | − | + | + | − |
| ORF-CYFP + NYFP-ORF | − | − | + | + | − |
| CYFP-ORF + ORF-NYFP | + | − | + | + | − |
| CYFP-ORF + NYFP-ORF | − | + | + | + | − |
| Negative controls | |||||
| CYFP-ORF + NYFP-cyt | − | − | − | − | − |
| ORF-CYFP + NYFP-cyt | − | − | − | − | − |
| NYFP-ORF + CYFP-ER | − | − | − | − | − |
| P/N OFV | P/MP OFV | p29 CiLV-C/p29 CiLV-C2 | MP CiLV-C/MP CiLV-C2 | p29 CiLV-C/MP CiLV-C2 | |
| ORF-NYFP + ORF-CYFP | − | + | + | − | − |
| ORF-NYFP + CYFP-ORF | + | + | + | − | − |
| NYFP-ORF + ORF-CYFP | + | − | + | − | − |
| NYFP-ORF + CYFP-ORF | + | + | + | − | − |
| ORF-CYFP + ORF-NYFP | + | + | + | − | + |
| ORF-CYFP + NYFP-ORF | + | + | + | − | − |
| CYFP-ORF + ORF-NYFP | + | + | + | − | + |
| CYFP-ORF + NYFP-ORF | + | + | + | − | − |
| Negative controls | |||||
| CYFP-ORF + NYFP-cyt | − | − | − | − | − |
| ORF-CYFP + NYFP-cyt | − | − | − | − | − |
| NYFP-ORF + CYFP-ER | − | − | − | − | − |
| p29 CiLV-C2/MP CiLV-C | N CiLV-N/N OFV | MP CiLV-N/MP OFV | N CiLV-N/MP OFV | N OFV/MP CiLV-N | |
| ORF-NYFP + ORF-CYFP | − | + | − | − | − |
| ORF-NYFP + CYFP-ORF | + | + | − | − | + |
| NYFP-ORF + ORF-CYFP | − | − | + | − | − |
| NYFP-ORF + CYFP-ORF | − | + | − | − | − |
| ORF-CYFP + ORF-NYFP | + | − | + | − | − |
| ORF-CYFP + NYFP-ORF | + | − | + | − | − |
| CYFP-ORF + ORF-NYFP | + | + | + | − | − |
| CYFP-ORF + NYFP-ORF | − | − | + | − | + |
| Negative controls | |||||
| CYFP-ORF + NYFP-cyt | − | − | − | − | − |
| ORF-CYFP + NYFP-cyt | − | − | − | − | − |
| NYFP-ORF + CYFP-ER | − | − | − | − | − |
| Intra-association control | |||||
| MP CiLV-C2/MP OFV | p29 CiLV-C2/N OFV | p29 CiLV-C2/MP OFV | MP CiLV-C2/N OFV | MP CiLV-C/p24 CiLV-C | |
| ORF-NYFP + ORF-CYFP | + | + | − | − | − |
| ORF-NYFP + CYFP-ORF | − | + | − | − | − |
| NYFP-ORF + ORF-CYFP | + | − | − | − | − |
| NYFP-ORF + CYFP-ORF | − | − | + | − | − |
| ORF-CYFP + ORF-NYFP | − | + | + | − | − |
| ORF-CYFP + NYFP-ORF | − | + | + | − | − |
| CYFP-ORF + ORF-NYFP | + | + | + | − | − |
| CYFP-ORF + NYFP-ORF | − | + | − | − | − |
| Negative controls | |||||
| CYFP-ORF + NYFP-cyt | − | − | − | − | − |
| ORF-CYFP + NYFP-cyt | − | − | − | − | − |
| NYFP-ORF + CYFP-ER | − | − | − | − | − |
| Inter-association controls | |||||
| CP AMV/MP CiLV-C | N TSWV/MP CiLV-C | ||||
| ORF-NYFP + ORF-CYFP | − | − | |||
| ORF-NYFP + CYFP-ORF | − | − | |||
| NYFP-ORF + ORF-CYFP | − | − | |||
| NYFP-ORF + CYFP-ORF | − | − | |||
| ORF-CYFP + ORF-NYFP | n.d | − | |||
| ORF-CYFP + NYFP-ORF | n.d | − | |||
| CYFP-ORF + ORF-NYFP | n.d | − | |||
| CYFP-ORF + NYFP-ORF | n.d | − | |||
| Negative controls | |||||
| CYFP-ORF + NYFP-cyt | − | − | |||
| ORF-CYFP + NYFP-cyt | − | − | |||
| NYFP-ORF + CYFP-ER | − | − | |||
The symbols − and + correspond to the absence and presence of fluorescence signals, respectively. NYFP and CYFP correspond to half N- and C-terminal of the YFP protein, respectively. OFV = OFV-citrus. n.d = non-determined. Additional negative controls are presented for intra- and inter-association assays.