Fig. 2.

Titers of p-coumaric acid, resveratrol, cis,cis-muconic acid and salicylic in engineered Y. lipolytica strains. Cultivations were carried out for 72 h in 24 deep-well plates containing mineral medium with 20 g/L glucose. Extracellular content was subjected to HPLC analysis. Error bars represent standard deviation from at least three biological replicates. “-” and “+” symbols indicate absence or presence of the corresponding genetic modification, respectively. a) Production of p-coumaric acid and resveratrol with supplementation of 2 mM L-tyrosine. b) Degradation assays for p-coumaric acid and resveratrol in strain ST6512, at different supplementation concentrations. “-” represents a medium control, non-inoculated. c) Production of cis,cis-muconic acid and the pathway intermediate protocatechuic acid (PCA). d) Production of salicylic acid. Statistical analysis was performed using Student's t-test (two-tailed; *P ≤ 0.05 two-sample unequal variance).