Fig. 4.

Effect on resveratrol titers of the integration of additional copies of the heterologous pathway. Cultivations were carried out for 72 h in 24 deep-well plates containing mineral medium with 20 g/L glucose. Extracellular content was subjected to HPLC analysis. “-” and “+” symbols indicate absence or presence of the corresponding genetic modification, respectively. Digits show the number of additional copies of resveratrol biosynthetic genes integrated. Error bars represent standard deviation from at least three biological replicates. p-Coumaric acid was not detected in any of the strains.